LentiORF particles available

• Coverage: Genome-wide, Human, Mouse & Rat
• Ready-to-use: Just add to cells and get gene expression
• Broad spectrum: transduce dividing, non-dividing and in vivo
• Biosafety: replication deficient 3rd generation

TrueORF cDNA clones are tagged ORF clones in mammalian expression vectors

• Genome wide coverage for human, mouse and rat
• Two types of tags: Myc-DDK tagged or GFP tagged
• New: Lenti options are now available.
• 20,000 Human clones are validated for protein expression
• 70 destination vectors for additional applications
• Clone collections available

The same ORF inserts are available in 4 different expression vectors

Technical features of TrueORF:

• The CMV promoter and a Kozak consensus sequence drive protein expression in mammalian cells.
• The C-terminus of the ORF is tagged with Myc/DDK* or tGFP.
• Ten (10) ug of purified plasmid is provided for immediate transfection and expression experiments.
• The upstream T7 promoter allows protein expression in cell-free system.
• Engineered as the entry vector for the OriGene PrecisionShuttle system.

  Technical features of TrueORF:

  • The CMV promoter and a Kozak consensus sequence drive protein expression in mammalian cells.
  • The C-terminus of the ORF is tagged with Myc/DDK* or tGFP.
  • Ten (10) ug of purified plasmid is provided for immediate transfection and expression experiments.
  • The upstream T7 promoter allows protein expression in cell-free system.
  • Engineered as the entry vector for the OriGene PrecisionShuttle system.

Each TrueORF Clone is provided with a pair of sequencing primers: